Coding
CDA

Part:BBa_K1266000:Design

Designed by: Janet Standeven   Group: iGEM14_Lambert_GA   (2014-06-22)


Chitin Deacetylase or CDA2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 410
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 521


Design Notes

One illegal restriction site had to be removed from the coding sequence. This is the entire coding region. Some literature suggests that 140bps may be removed and still maintain protein function. This has not been verified in this particular part.


Source

S. cerevisiae 288c

References